Kinetic characterization of acetone monooxygenase from Gordonia sp. strain TY-5
نویسندگان
چکیده
منابع مشابه
THE GENE CLONING, OVEREXPRESSION, PURIFICATION, AND CHARACTERIZATION OF DIBENZOTHIOPHENE MONOOXYGENASE AND DESULFINASE FROM GORDONIA ALKANIVORANS RIPI90A
The biodesulfurization (BDS) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. Gordonia alkanivorans RIPI90A is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-HBP) in 4S pathway. In this study, (DBT), DszA and DszB, DBT sulfone monooxygenase, and desulfinase were respectivel...
متن کاملPurification and characterization of 2,4,6-trichlorophenol-4-monooxygenase, a dehalogenating enzyme from Azotobacter sp. strain GP1.
The enzyme which catalyzes the dehalogenation of 2,4,6-trichlorophenol (TCP) was purified to apparent homogeneity from an extract of TCP-induced cells of Azotobacter sp. strain GP1. The initial step of TCP degradation in this bacterium is inducible by TCP; no activity was found in succinate-grown cells or in phenol-induced cells. NADH, flavin adenine dinucleotide, and O2 are required as cofacto...
متن کاملBiochemical characterization of StyAB from Pseudomonas sp. strain VLB120 as a two-component flavin-diffusible monooxygenase.
Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) and a NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type Pseudomonas strain VLB120 as well as from recombinant Escherichia coli. StyA from both sources is a dimer, with a subunit size of 47 kDa, and catalyzes th...
متن کاملthe gene cloning, overexpression, purification, and characterization of dibenzothiophene monooxygenase and desulfinase from gordonia alkanivorans ripi90a
the biodesulfurization (bds) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. gordonia alkanivorans ripi90a is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-hbp) in 4s pathway. in this study, (dbt), dsza and dszb, dbt sulfone monooxygenase, and desulfinase were respectivel...
متن کاملThe Gene Cloning, Overexpression, Purification, and Characterization of Dibenzothiophene Monooxygenase and Desulfinase from Gordonia Alkanivorans Ripi90a
The biodesulfurization (BDS) of sulfur compounds in fossil fuels is a process to reduce sulfur dioxide emissions that cause environmental pollution. Gordonia alkanivorans RIPI90A is able to convert dibenzothiophene, an organic sulfur compound in petroleum, to 2-hydroxybiphenyl (2-HBP) in 4S pathway. In this study, (DBT), DszA and DszB, DBT sulfone monooxygenase, and desulfinase were respectivel...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2018
ISSN: 2191-0855
DOI: 10.1186/s13568-018-0709-x